Table 3. Media weak-electrolyte-component molarities and computed percentages of proton flux (J%) by media component. Comparison of original media with media modified for examination of proton flux measurement.

Media, pH

components

[B]

mM

J%

references

Landureau's, pH 6.8

H+

glu

gly

his

lys

H2PO3

 

-

10

10

1.3

1.4

11

 

0.06

1.2

0.5

5.3

0.3

92.1

 

 

 

 

 

insect tissue culture medium [11]

Landureau's(-aa), pH 6.8

H+

H2PO3

 

-

1

 

0.7

99.3

 

modified for proton flux measurements [10]

DMA, 6.5

H+

glu

gly

his

lys

met

PIPES

H3PO4

 

-

10

10

1.2

1.4

1.7

1

1

 

0.52

6.55

1.09

33.28

0.67

0.43

24.16

32.21

 

 

 

 

 

 

 

Achlya growth medium [12]

DMA (deficient), 6.5

H+

glu

met

PIPES

H3PO4

 

-

1

1

1

1

 

0.90

0.86

0.43

41.5

55.4

 

 

 

 

Achlya medium for proton flux study [12]