Table 3.
Media weak-electrolyte-component molarities and computed percentages of proton flux (J%) by media component. Comparison of original media with media modified for examination of proton flux measurement.
Media, pH components |
[B] mM |
J% |
references |
Landureau's, pH 6.8 H+ glu gly his lys H2PO3 |
- 10 10 1.3 1.4 11 |
0.06 1.2 0.5 5.3 0.3 92.1 |
insect tissue culture medium [11] |
Landureau's(-aa), pH 6.8 H+ H2PO3 |
- 1 |
0.7 99.3 |
modified for proton flux measurements [10] |
DMA, 6.5 H+ glu gly his lys met PIPES H3PO4 |
- 10 10 1.2 1.4 1.7 1 1 |
0.52 6.55 1.09 33.28 0.67 0.43 24.16 32.21 |
Achlya growth medium [12] |
DMA (deficient), 6.5 H+ glu met PIPES H3PO4 |
- 1 1 1 1 |
0.90 0.86 0.43 41.5 55.4 |
Achlya medium for proton flux study [12] |