Table 2.
The buffer molarities used by investigators in non-invasive ion probe studies of biological systems with a computed facilitation factor (DF/TF) of increased proton flux due to that level of buffer at the prevailing or potential pHs.|
Buffer pK |
molarities, pH |
D F/TF |
references |
|
MES 6.15 |
15 mM, pH 5.5 1 mM, pH 6.0 50 µM, pH 6.15 |
63 22 3 |
pollen tube growth pollen tube growth and Ca flux [9] pollen tube growth and H flux [4] |
|
HEPES 7.55 |
0.001, pH 8.2 0.001, pH 7.2 |
1495 230 |
gastric oxyntic cells [5] Periplaneta oocytes [10] |
|
Bicarbonate 6.13 |
0.005, pH 8.2 0.0024, pH 7.8 0.0048, pH 7.8 0.0024, pH 6.13 |
840 395 789 104 |
gastric oxyntic cells [5] Xenopus oocytes [10] |
|
Boric acid 9.14 |
0.0016, pH 6.0 0.0016, pH 7.0 0.0016, pH 8.0 0.0016, pH 8.2 |
1 16 1281 2976 |
pollen tube growth Ca flux [9] and H flux [4] |