ZEBRAFISH AS AN ANIMAL MODEL FOR DEVELOPMENT AND DISEASE RESEARCH - NIH


Release Date:  May 21, 1998

PA NUMBER:  PA-98-074

P.T.

National Institute of Child Health and Human Development
National Institute of Diabetes and Digestive and Kidney Diseases
National Cancer Institute
National Center for Research Resources
National Eye Institute
National Heart, Lung, and Blood Institute
National Human Genome Research Institute
National Institute on Aging
National Institute of Alcohol Abuse and Alcoholism
National Institute of Allergy and Infectious Diseases
National Institute of Arthritis and Musculoskeletal and Skin Diseases
National Institute on Deafness and Other Communication Disorders
National Institute of Dental Research
National Institute on Drug Abuse
National Institute of Environmental Health Sciences
National Institute of General Medical Sciences
National Institute of Mental Health
National Institute of Neurological Disorders and Stroke

PURPOSE

The purpose of this Program Announcement (PA) is to solicit applications
as part of a National Institutes of Health (NIH) initiative to increase
our support of the zebrafish as an animal model for research.  This PA
is intended to continue stimulation of a trans-NIH initiative that was
started with RFA: DK-98-006, entitled "Genomic Resources for the
Zebrafish," NIH Guide for Grants and Contracts, Vol. 26, No. 39,
December 5, 1997.

HEALTHY PEOPLE 2000

The Public Health Service (PHS) is committed to achieving the health
promotion and disease prevention objectives of "Healthy People 2000," a
PHS-led national activity for getting priority areas.  Potential
applicants may obtain a copy of "Healthy People 2000" (Full Report:
Stock No. 017-001-00474-0 or Summary Report: Stock No. 017-001-00473-1)
through the Superintendent of Documents, Government Printing Office,
Washington, DC 20402-9325 (telephone: 202-512-1800).

ELIGIBILITY REQUIREMENTS

Applications may be submitted by domestic and foreign for-profit and
non-profit organizations, public and private, such as universities,
colleges, hospitals, laboratories, units of State and local governments,
and eligible agencies of the Federal government.  Racial/ethnic minority
individuals, women, and persons with disabilities are encouraged to
apply as Principal Investigators.

MECHANISM OF SUPPORT

The mechanism of support for this PA will be the NIH investigator-
initiated research project grant (R01) award.  Applications for R01s
from new investigators are particularly encouraged.  The total project
period for an application submitted in response to this PA may not
exceed five years.

Responsibility for the planning, direction, and execution of the
proposed project will be solely that of the applicant.  Applications
will compete with all investigator-initiated applications and will be
reviewed according to the customary peer review procedures. Awards will
be administered under PHS grants policy as stated in the Public Health
Service Grants Policy Statement.

This PA is the result of a trans-NIH initiative with participation of
the Institutes and Centers listed above, working though the Cross-NIH
Zebrafish Coordinating Committee, under the co-chairmanship of the
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
and the National Institute of Child Health and Human Development
(NICHD).  The principal awards will be made through the Institute or
Center whose mission is most closely related to the proposed work.
Through the Cross-NIH Zebrafish Coordinating Committee, each Institute
will share with the other participating Institutes, research supported
as a result of this PA . All investigators funded under this initiative
will be expected to work together cooperatively so that the information
learned and the models developed will be of maximum usefulness to the
community.

An applicant planning to submit a grant application requesting $500,000
or more in direct costs for any year is required to contact, in writing
or by telephone, Institute or Center program staff when the application
development process begins.  Furthermore, the applicant must obtain
agreement from Institute/Center staff that the Institute or Center will
accept the application for consideration for award.  The applicant
Principal Investigator must identify, in a cover letter sent with the
application, the program staff member and Institute or Center that has
agreed to accept assignment of the application.  An application received
without indication of prior staff concurrence and identification of that
contact will be returned to the applicant without review.

RESEARCH OBJECTIVES

Background

Vertebrate development has been characterized extensively using the
methods of classical embryology, molecular biology and biochemistry.
However, mutational analysis in vertebrates, has lagged behind such
investigations in invertebrates. As experimentation in Drosophila
melanogaster and Caenorhabditis elegans has established, mutational
studies are a powerful tool to determine the events that result in
patterning and morphogenesis of the embryo.  When combined with genetic
combinatorial analyses, mutational analyses can identify specific genes
that act during embryonic development, provide insight into how they
function, and clarify the pathways in which they participate.  Studies
that compare results from these invertebrate systems with those obtained
in vertebrates have established that there is remarkable evolutionary
conservation in the genetic programs that determine embryo formation,
including such early patterning events as formation of the embryonic
axes, but also including later events such as development of eye, heart,
and other organs.  Although invertebrate systems are extremely powerful
and numerous aspects of development are conserved, many aspects of
patterning and morphogenesis of the vertebrate embryo are distinct and
cannot be studied in invertebrates.  The vertebrate embryo has many
features not present in other models, including the substantially
different organization and greater complexity of the nervous system and
the fact that some vertebrate organs have no clear cognates in the
simpler invertebrates.  Thus, understanding human development will
require application of experimental approaches to the formation of the
vertebrate embryo.  Some assessment of mutations that affect development
has been possible in the mouse, but the mouse embryo is inaccessible in
utero throughout much of its development.  Consequently, mutational
studies in this species have been limited largely to defects in post-
natal maturation.  While reverse genetics (e.g., knock-outs) have been
useful in the mouse model, the substantial costs of maintaining large
mouse colonies has limited the applicability of forward genetic
approaches, which will have a profound impact on our understanding of
development.

As a vertebrate, the zebrafish, Danio rerio, is more closely related to
humans than are yeast, worms or flies.  It has a number of valuable
features as a model organism for study of vertebrate development.  Many
features of zebrafish development have been characterized, including
early embryonic patterning, early development of the nervous system, and
aspects of cell fate and lineage determination.  The embryos are
transparent and accessible throughout development.  In live embryos, the
same specific cell or even cellular processes can in many cases be
identified from individual to individual, affording a high level of
precision in characterizing the effect of developmental or genetic
perturbation.  Techniques for ablation and transplantation of individual
cells have been used to explore questions about induction and cell fate,
and continue to be refined.  There are also a growing number of
molecular markers to facilitate developmental studies.  Because of their
relatively short reproductive cycle, the large number of progeny that
can be produced, and the relatively small space needed to maintain large
numbers of offspring, the zebrafish is an efficient vertebrate model
system for genetic analysis.  It is possible to generate haploid
progeny, which are viable to the point where many recessive embryonic
phenotypes can be identified, and also homozygous diploid progeny that
carry only maternal (or paternal) genetic information.  A genetic map of
approximately 2-3 centimorgan resolution is available, and mutations can
be readily placed on the map.  Positional cloning of genes identified by
mutation has recently been accomplished.  Finally, there are several
promising methods for transformation and insertional mutagenesis which
are now being developed.

Two large-scale screens have been performed to date and the transparent
embryos screened for defects in overall embryonic pattern, morphogenesis
or organ formation.  As reported in the December 1996 issue of
Development, these screens have identified a substantial number of
mutations that affect the formation of organ systems, including defects
in the nervous system, skeletal muscle, craniofacial region, kidney and
endocrine organs, cardiovascular and gastrointestinal systems, and the
sensory cells of lateral line systems which are important to auditory
and vestibular function.  For most of these mutations, the gene defect
has not yet been identified.  It is likely that many of these mutations
affect genes relevant to human development and disease processes such as
cancer and neurodegenerative diseases.  The zebrafish offers the
opportunity of using classical genetics to define gene functions.

Scope

The objective of this PA is to promote the zebrafish as an animal model
for the study of development and disease.  The goals of this PA are to
encourage new and innovative research and approaches using the zebrafish
to identify the genes and elucidate the molecular and genetic mechanisms
responsible for normal and defective development and disease.

Each of the participating Institutes and Centers has interests in using
the zebrafish as a model system to better understand particular
processes, organs, or diseases.  In addition, some may be interested in
supporting development of methods, either general techniques or
techniques that may particularly apply to their areas of interest.  Each
Institute with their general statement of interest is listed below in
alphabetical order.  Some Institutes have included examples of research
topics that are appropriate for this PA; however, they are not to be
considered as exclusive or limiting.

Institute or Center Statement of Interest

NCI.  Generation and study of zebrafish models to identify and place
genes in functional pathways that affect growth and development; in
particular, genes/pathways which, when altered, result in uncontrolled
or cancerous growth.

NCRR.  Research projects to broaden the utility of the zebrafish model.
Projects should be applicable to a broad range of biomedical research
and to more than one categorical NIH Institute or specific disease.

NEI.  Fundamental mechanisms underlying all aspects of eye development,
function and disease, including retinal development and optic axon
guidance.

NHGRI.  Proposals for the development of high throughput, widely
applicable technologies or methodologies to examine gene function on a
genomic scale.  This could include initial development of high
throughput or large-scale methods for examining gene expression,
development of tools for comprehensive mutational analysis, or genome-
scale identification of regulatory regions.

NHLBI.  Cellular and molecular functions of the mutant genes in
development as models for human cardiovascular, blood and pulmonary
disorders, and circadian mechanisms underlying sleep/wake cycles.
Genetic basis of disorders of cardiovascular development and function;
developmental aspects of endothelial dysfunction as the basis for
systemic and pulmonary vascular disorders; developmental defects in
hematopoiesis and relationship to disorders of the hematopoietic system;
genetic basis of vasculogenesis; effect of mutations on subsequent organ
development leading to such disorders as lung hypoplasia and
bronchopulmonary dysplasia; and the genetic basis of circadian rhythm.

NIA.  Basic research on the genetic and molecular basis of aging and
longevity. Generation and analysis of late-age onset or long-lived
mutants that can be used to identify, clone, and characterize genes
involved in normal and pathological aging.  Cellular and molecular
function of genes expressed in the aging nervous system as well as in
other organ systems, such as the cardiovascular, immune, and
musculoskeletal systems.  Such genes include, but are not limited to,
those involved in neurodegenerative disorders, neuroplasticity, cell
death, damage and repair of DNA and proteins, and oxidative metabolism,
and maintenance of differentiated cell function.

NIAAA.  Mechanistic studies of ethanol-induced teratogenesis, behavioral
impairments, and organ damage.

NIAID.  Mechanisms underlying immune system development and function.
Hematopoiesis, including the development of T cells, B cells, antigen
presenting cells, and cells of the innate immune system, such as natural
killer cells. Regulation of key immune cell molecules during
development, such as regulation of genes encoding antigen receptors, co-
receptors, cytokine receptors, and cytokines.  Regulation of genes
involved in mature immune cell function following antigen encounter,
including regulation and role of cytokines, receptor expression, and any
other determinants of humoral and cellular immunity following antigen
administration.

NIAMS.  Generation and analysis of mutant zebrafish that have the
potential to illuminate the development and function of the vertebrate
musculoskeletal system and skin.  The musculoskeletal system includes
muscle, bone, articulated joints, cartilage, tendon and ligament.
Priority will be given to the establishment of collaborations between
investigators with expertise in the zebrafish and investigators with
expertise in the musculoskeletal systems and skin of mammals and humans.

NICHD.  Identification, cloning, and characterization of the genes
important in normal development as well as those mutant genes which
cause developmental defects.  Elucidation of the cellular, biochemical,
molecular and genetic mechanisms underlying normal and defective
development.  This includes, but is not limited to the study of general
mechanisms of pattern formation and cell lineage, cell specification,
differentiation, migration and fate in early development of many
organs/systems.

NIDA.  Identification of mechanisms underlying tolerance, sensitization,
and addiction to drugs of abuse such as nicotine, amphetamine, cocaine,
opiates, barbiturates, and hallucinogens.  Identification of genetic
suppressors and enhancers of the teratological effects of drugs of abuse
on behavior and the nervous system.  Processes involved in the
development of brain regions mediating the hedonic properties of drugs
of abuse.

NIDCD.  Identification and cloning of genes involved in the normal and
disordered development of hearing, balance, smell and taste sensory
systems.  Elucidation of the cellular, molecular, and biochemical
mechanisms governing the proliferative, plastic and regenerative
capacities of these sensory cells and tissues.

NIDDK.  Research on diabetes, other endocrine and metabolic diseases,
disorders of hematopoeisis, and for diseases of the digestive system,
liver, kidney, and urinary tract.  Studies aiming to clarify the
cellular and molecular events which dictate organ formation in all these
systems are considered of relevance.  These studies could include but
need not be limited to studies to develop cell lines from any of the
organs of interest, studies to characterize normal or abnormal function
of organs of interest, methods to screen and identify additional
mutations in these systems, studies to define the molecular mechanisms
which dictate cell-specific gene expression in relevant cell types.

NIDR.  All aspects of normal and abnormal craniofacial development,
including genetics, complex origins of craniofacial disorders, cell
lineages and differentiation, cell signaling and gene regulation,
embryonic patterning, imaging, biomimetics, and new technologies for
high-throughput genetic and protein screens.

NIEHS.  Studies to examine the mechanism whereby environmental
factors/agents alter development.  Characterization of the interactions
among genetics, environmental agents and time during development that
lead to structural or functional abnormalities.  Development of a
mechanistically based model for testing environmental agents for
developmental toxicity.

NIGMS.  Basic biomedical research that addresses fundamental biological
mechanisms such as those that underlie gene regulation, chromosome
organization and mechanics, cell growth and differentiation, pattern
formation, sex determination, morphogenesis, cell cycle control,
behavior, the genetics of complex traits, and the application of
mathematical models to complex biological systems.

NIMH.  Investigations which examine molecular, cellular, and biochemical
bases of genetic mutations affecting neurogenesis and mental behavior.
These studies include, but are not limited to, screening for such
mutants, identification, isolation, and mapping of the defective genes,
and their functional analyses.

NINDS.  Research on the development, normal function, and diseases of
the nervous system.  This research might include the use of mutants to
understand the mechanisms controlling the following processes:
neurogenesis, nervous system patterning, cell lineage, cell migration,
programmed cell death, axon pathfinding and regeneration, myelination,
neural crest cell development, and motor and sensory function.  Also of
interest would be research into the functional neuroanatomy of
developing and adult nervous systems, the use of optical imaging
techniques to visualize neural activity and development of methods for
analyzing and perturbing gene function in single cells.

The areas of interest listed above are not in any order of priority.
They are only examples of areas of research to consider.  Applications
with areas of interest to more than one Institute or Center will be
assigned to multiple Institutes or Centers for funding consideration.
Applicants are encouraged to propose other areas that are related to the
objectives and scope of this PA.

APPLICATION PROCEDURES

Applications are to be submitted on the grant application form PHS 398
(rev. 5/95) and will be accepted on the standard application deadlines
as indicated in the application kit.  Requests for continued funding of
already funded projects (Type 2) will be considered under this program
announcement.  Application kits are available at most institutional
offices of sponsored research and may be obtained from the Division of
Extramural Outreach and Information Resources, National Institutes of
Health, 6701 Rockledge Drive, MSC 7910, Bethesda, MD 20892-7910,
telephone 301/435-0714, email: Grantsinfo@nih.gov.

For identification and processing purposes, the PA number and title must
be typed in item 2 of the application face page and the "YES" box must
be marked.

Submit a signed, typewritten original of the application, including the
Checklist, plus five signed photocopies, in one package to:

CENTER FOR SCIENTIFIC REVIEW
NATIONAL INSTITUTES OF HEALTH
6701 ROCKLEDGE DRIVE, ROOM 1040 - MSC 7710
BETHESDA, MD  20892-7710
BETHESDA, MD  20817 (for express/courier service)

REVIEW CONSIDERATIONS

Applications will be assigned on the basis of established PHS referral
guidelines.  Upon receipt, applications will be reviewed for
completeness by Center for Scientific Review (CSR).  Incomplete
applications will be returned to the applicant without further
consideration.  Applications will be evaluated for scientific and
technical merit by an appropriate peer review group convened in
accordance with the standard NIH peer review procedures.  As part of the
initial merit review, all applications will receive a written critique
and undergo a process in which only those applications deemed to have
the highest scientific merit, generally the top half of applications
under review, will be discussed, assigned a priority score, and receive
a second level review by the appropriate national advisory council or
board.

Review Criteria

The goals of NIH-supported research are to advance our understanding of
biological systems, improve the control of disease, and enhance health.
In the written review, comments on the following aspects of the
application will be made in order to judge the likelihood that the
proposed research will have a substantial impact on the pursuit of these
goals.  Each of these criteria will be addressed and considered in the
assignment of the overall score.

o  Significance: scientific, technical or medical significance and
originality of the proposed research will be evaluated.

o  Approach: Are the conceptual framework, design, methods, and analyses
appropriate and adequate to accomplish the aims of the project?  Does
the applicant acknowledge potential problem areas and consider
alternative tactics? Is the scientific and technical merit of the
research proposed sufficient to advance the objectives of the PA?

o  Innovation: Does the project employ novel concepts, approaches or
method? Are the aims original and innovative?  Does the project
challenge existing paradigms or develop new methodologies or
technologies?

o  Investigator: Are the Principal Investigator and staff appropriately
trained and well suited to carry out this work?  Is the work proposed
appropriate to the experience level of the principal investigator and
other researchers (if any)?

o  Environment:  Does the scientific environment in which the work will
be done contribute to the probability of success?  Do the proposed
experiments take advantage of unique features of the scientific
environment or employ useful collaborative arrangements?  Is there
evidence of institutional support?

o  Budget and duration: Are the proposed budget and duration appropriate
in relation to the proposed research?

Availability of special opportunities for furthering research programs
through the use of unusual talent resources, populations, or
environmental conditions in other countries that are not readily
available in the United States or that provide augmentation of existing
U.S. resources will be considered in the review.

The initial review group also will examine the provisions for the
protection of human and animal subjects, and the safety of the research
environment.

AWARD CRITERIA

Factors that will be used to make award decisions are as follows:

o  Quality of the proposed project as determined by peer review;

o  Cost effectiveness of the proposed strategy;

o  Promise of the proposed program to accomplish the goals of this PA
and address the needs of the participating ICs as regards their interest
in the zebrafish as a model organism;

o  Availability of funds.

INQUIRIES

Written and telephone inquiries concerning this PA are encouraged.  The
opportunity to clarify any issues or questions from potential applicants
is welcome.

Direct inquiries regarding programmatic issues to:

Dr. Deborah Henken
Center for Research for Mothers and Children
National Institute of Child Health and Human Development
6100 Executive Boulevard, Room 4B01 MSC 7510
Bethesda, MD  20892-7510
Telephone:  (301) 496-5541
FAX:  (301) 402-4083
Email:  dh50g@nih.gov

Dr. David G. Badman
Division of Kidney, Urologic and Hematologic Diseases
National Institute of Diabetes and Digestive and Kidney Diseases
45 Center Drive, Room 6AS-13C MSC 6600
Bethesda, MD  20892-6600
Telephone:  (301) 594-7717
FAX:  (301) 480-3510
Email:  David_Badman@nih.gov

Dr. Grace L. Shen
Division of Cancer Biology
National Cancer Institute
6130 Executive Boulevard, Room 501
Rockville, MD  20892-7381
Telephone:  (301) 435-5226
FAX:  (301) 496-8656
Email:  gs35r@nih.gov

Dr. Jill L. Carrington
Comparative Medicine
National Center for Research Resources
6705 Rockledge Drive, Room 6164 MSC 7965
Bethesda, MD  20892-7965
Telephone:  (301) 435-0776
FAX:  (301) 480-3659
Email: jillc@ep.ncrr.nih.gov

Dr. Maria Y. Giovanni
Fundamental Retinal Processes
National Eye Institute
Executive Plaza South, Suite 350 - MSC 7164
Bethesda, MD  20892-7164
Telephone:  (301) 496-0484
FAX:  (301) 402-0528
Email:  myg@nei.nih.gov

Dr. Carol H. Letendre
Division of Blood Diseases and Resources
National Heart, Lung, and Blood Institute
6701 Rockledge Drive, MSC 7950
Bethesda, MD  20892-7950
Telephone:  (301) 435-0080
FAX:  (301) 480-0867
Email:  letendrc@gwgate.nhlbi.nih.gov

Dr. Adam Felsenfeld
National Human Genome Research Institute
38 Library Drive, Room614, MSC 6050
Bethesda, MD  20892-6050
Telephone:  (301) 496-7531
FAX:  (301) 480-2770
Email:  felsenfa@odder.nhgri.nih.gov

Dr. Bradley C. Wise
Neuroscience and Neuropsychology of Aging Program
National Institute on Aging
7201 Wisconsin Avenue, Room 3C307, MSC 9205
Bethesda, MD  20892-9205
Telephone:  (301) 496-9350
FAX:  (301) 496-1494
Email:  bw86y@nih.gov

Dr. Robert W. Karp
Division of Basic Research
National Institute on Alcohol Abuse and Alcoholism
6000 Executive Boulevard, Suite 402, MSC 7003
Bethesda, MD  20892-7003
Telephone:  (301) 443-2239
FAX:  (301) 594-0673
Email:  rkarp@willco.niaaa.nih.gov

Dr. Vicki Seyfert
Division of Allergy, Immunology and Transplantation
National Institute on Allergy and Infectious Disease
6003 Executive Boulevard, Room 4A21, MSC 7610
Bethesda, MD  20892-7610
Telephone:  (301) 496-7551
FAX:  (301) 402-2571
Email:  vs62y@nih.gov

Dr. Richard W. Lymn
Muscle Biology Branch
National Institute on Arthritis and Musculoskeletal and Skin Diseases
45 Center Drive, Room 5AS-49E, MSC 6500
Bethesda, MD  20892-6500
Telephone:  (301) 594-5130
FAX:  (301) 480-4543
Email:  r128b@nih.gov

Dr. Chyren Hunter
Division of Human Communication
National Institute on Deafness and Other Communication Disorders
6120 Executive Boulevard, Room 400-C, MSC 7180
Bethesda, MD  20982-7180
Telephone:  (301) 402-3461
FAX:  (301) 402-6251
Email:  chyren_hunter@nih.gov

Dr. Norman Braveman
Inherited Diseases and Disorders Program
National Institute of Dental Research
45 Center Drive, Room 4AN24, MSC 6402
Bethesda, MD  20892-6402
Telephone:  (301) 594-2089
FAX:  (301) 480-8318
Email:  Bravemann@de45.nidr.nih.gov

Dr. Jonathan D. Pollock
Division of Basic Research
National Institute on Drug Abuse
5600 Fishers Lane, Room 10A19
Rockville, MD  20857
Telephone:  (301) 443-6300
FAX:  (301) 594-6043
Email:  jp183r@nih.gov

Dr. Jerrold J. Heindel
Division of Extramural Research and Training
National Institute of Environmental Health Sciences
P.O. Box 12233 (md EC-23)
Research Triangle Park, NC  27709
Telephone:  (919) 541-0781
FAX:  (919) 541-5064
Email:  heindelj@niehs.nih.gov

Dr. Judith H. Greenberg
Division of Genetics and Developmental Biology
National Institute of General Medical Sciences
45 Center Drive, MSC 6200
Bethesda, MD  20892-6200
Telephone:  (301) 594-0943
FAX:  (301) 480-2228
Email:  greenbej@nigms.nih.gov

Dr. Hemin R. Chin
Division of Basic and Clinical Neuroscience Research
National Institute of Mental Health
5600 Fishers Lane, Room 10C-26
Rockville, MD  20857
Telephone:  (301) 443-1706
FAX:  (301) 443-9890
Email:  hemin@codon.nih.gov

Dr. Gabrielle G. Leblanc
Division of Fundamental Neurosciences and Developmental Disorders
National Institute of Neurological Disorders and Stroke
7550 Wisconsin Avenue, Room 8C08
Bethesda, MD  20892-9170
Telephone:  (301) 496-5745
FAX:  (301) 402-1501
Email:  gl54h@nih.gov

Direct inquiries regarding fiscal and administrative matters to:

Mr. E. Douglas Shawver
Grants Management Branch
National Institute of Child Health and Human Development
6100 Executive Boulevard, Room 8A17, MSC 7510
Bethesda, MD  20892-7510
Telephone:  (301) 496-1303
FAX:  (301) 402-0915
Email:  Shawverd@exchange.nichd.nih.gov

Ms. Aretina Perry-Jones
Division of Extramural Activities
National Institute of Diabetes and Digestive and Kidney Diseases
45 Center Drive, Room 6AN-38B, MSC 6600
Bethesda, MD  20892-6600
Telephone:  (301) 594-8862
FAX:  (301) 480-3504
Email:  PerryA@extra.niddk.nih.gov

Mr. Bill Wells
Grants Administration Branch
National Cancer Institute
6120 Executive Boulevard, Room 243
Bethesda, MD  20892
Rockville, MD  20852 (express/courier service)
Telephone:  (301) 496-7800, ext. 250
FAX:  (301) 496-8601
Email:  ww14j@nih.gov

Ms. Louise Amburgey
Office of Grants Management
National Center for Research Resources
6705 Rockledge Drive, Room 6086 MSC 7965
Bethesda, MD  20892-7965
Telephone:  (301) 435-0844
FAX:  (301) 480-3777
Email:  louisem@ep.ncrr.nih.gov

Ms. Carolyn E. Grimes
Grants Management Branch
National Eye Institute
Executive Plaza South, Suite 350, MSC 7164
Bethesda, MD  20892-7164
Telephone:  (301) 496-5884
FAX:  (301) 496-9997
Email:  cegrimes@nei.nih.gov

Ms. Jane R. Davis
Division of Extramural Affairs
National Heart, Lung, and Blood Institute
6701 Rockledge Drive, MSC 7926
Bethesda, MD  20892-7926
Telephone:  (301) 435-0166
FAX:  (301) 480-3310
Email:  jane_davis@nih.gov

Ms. Jean Cahill
Grants Management Office
National Human Genome Research Institute
38 Library Drive, Room 613, MSC 6050
Bethesda, MD  20892-6050
Telephone:  (301) 402-0733
FAX:  (301) 402-1951
Email:  cahillj@odder.nhgri.nih.gov

Mr. Joseph Ellis
Grants Management Officer
National Institute on Aging
7201 Wisconsin Avenue, Suite 2N212, MSC 9205
Bethesda, MD  20892-9205
Telephone:  (301) 496-1472
FAX:  (301) 402-3672
Email:  ellisj@exmur.nia.nih.gov

Ms. Linda Hilley
Office of Planning and Resource Management
National Institute on Alcohol Abuse and Alcoholism
6000 Executive Boulevard, Suite 504, MSC 7003
Bethesda, MD  20892-7003
Telephone:  (301) 443-4703
FAX:  (301) 443-3891
Email:  Lhilley@willco.niaaa.nih.gov

Ms. Lesia Norwood
Grants Management Branch
National Institute on Allergy and Infectious Disease
6003 Executive Boulevard, Room 4B27, MSC 7610
Bethesda, MD  20892-7610
Telephone:  (301) 402-6581
FAX:  (301) 480-3780
Email:  Lnorwood@mercury.niaid.nih.gov

Ms. Sally A. Nichols
Grants Management Branch
National Institute of Arthritis and Musculoskeletal and Skin Diseases
45 Center Drive, Room 5AS-49F, MSC 6500
Bethesda, MD  20892-6500
Telephone:  (301) 594-3535
FAX:  (301) 480-5450
Email:  sn21q@nih.gov

Ms. Sharon Hunt
Grants Management Office
National Institute on Deafness and Other Communication Disorders
6120 Executive Boulevard, Room 400-B, MSC-7180
Bethesda, MD  20982-7180
Telephone:  (301) 402-0909
FAX:  (301) 402-1758
Email:  sh79f@nih.gov

Ms. Eileen Teng
Grants Management Office
National Institute of Dental Research
45 Center Drive, Room 4AN32J, MSC 6402
Bethesda, MD 20892-6402
Telephone:  (301) 594-4800
FAX:  (301) 402-1517
Email:  Eileen.Teng@nih.gov

Ms. Catherine Mills
Grants Management Branch
National Institute on Drug Abuse
5600 Fishers Lane,  Room 8A-54
Rockville, MD  20857
Telephone:  (301) 443-6710
FAX:  (301) 594-6847
Email:  cm108w@nih.gov

Mr. David L. Mineo
Division of Extramural Research and Training
National Institute of Environmental Health Sciences
P.O. Box 12233 (md EC-22)
Research Triangle Park, NC  27709
Telephone:  (919) 541-7628
FAX:  (919) 541-2860
Email:  mineo@niehs.nih.gov

Ms. Marcia Cohn
Grants Management Office
National Institute of General Medical Sciences
45 Center Drive, Room 2AN-44E, MSC 6200
Bethesda, MD  20892-6200
Telephone:  (301) 594-3918
FAX:  (301) 480-1969
Email:  cohnm@nigms.nih.gov

Ms. Diana S. Trunnell
Grants Management Branch
National Institute of Mental Health
5600 Fishers Lane, Room 7C-08
Rockville, MD  20857
Telephone:  (301) 443-2805
FAX:  (301) 443-6885
Email:  dt21a@nih.gov

Ms. Tina Carlisle
Grants Management Branch
National Institute of Neurological Disorders and Stroke
Federal Building, Room 1004
Bethesda, MD  20892-9190
Telephone:  (301) 496-9231
FAX:  (301) 402-0218
Email:  tc48k@nih.gov

AUTHORITY AND REGULATIONS

This program is described in the Catalog of Federal Domestic Assistance
No. 93.865 - Research for Mothers and Children, National Institute of
Child Health and Human Development.  Awards are made under authorization
of the Public Health Service Act, Title IV, Part A (Public Law 78-410,
as amended by Public Law 99-158, 42 USC 241 and 285) and administered
under PHS grants policies and Federal Regulations 42 CFR 52 and 45 CFR
Part 74. This program is not subject to the intergovernmental review
requirements of Executive Order 12372 or Health Systems Agency review.

The PHS strongly encourages all grant and contract recipients to provide
a smoke- free workplace and promote the non-use of all tobacco products.
In addition, Public Law 103-227, the Pro-Children Act of 1994, prohibits
smoking in certain facilities (or in some cases, any portion of a
facility) in which regular or routine education, library, day care,
health care or early childhood development services are provided to
children.  This is consistent with the PHS mission to protect and
advance the physical and mental health of the American people.


KEYWORDS:

Keycode Keyword
A000760 National Institute of Child Health and Human Development
A000750 National Institute of Diabetes and Digestive and Kidney Diseases
A000730 National Cancer Institute
A000768 National Center for Research Resources
A000770 National Eye Institute
A000773 National Heart,Lung,and Blood Institute
A000765 National Center for Human Genome Research
A000775 National Institute on Aging
A000778 National Institute on Alcohol Abuse and Alcoholism
A000710 National Institute of Allergy and Infectious Diseases
A000780 National Institute of Arthritis & Musculoskeletal & Skin Diseases
A000785 National Institute on Deafness & Other  Communication Disorders
A000740 National Institute of Dental Research
A000783 National Institute on Drug Abuse
A000720 National Institute of Environmental Health Sciences
A000788 National Institute of General Medical Sciences
A000790 National Institute of Mental Health
A000793 National Institute of Neurological Disorders and Stroke
1014003 Animal Research Policy
071500A All Disease Entities & Medical Problems & Behavior
0700000 Health & Safety, Medical & Biomedical Sciences
0715085 Digestive Diseases & Disorders
0795003 Disease Control
0715000 Disease Entities & Medical Problems
0755020 Disease Model
075500A All Methodologies & Procedures (Health & Safety & Medical)
0745027 Disease Prevention
077500A All Physiological & Development Process
1002052 Animal Development
071000A All Disciplines & Fields, Health & Safety, Medical & Biomedical
0775000 Physiological & Development Process
0780010 Biomedical Research Resources, Other
0710030 Biomedical Research, Multidisciplinary
0710000 Disciplines & Fields, Health & Safety, Medical & Biomedical
078000A All Research Resources (Health & Safety & Medical)
1002001 Anatomy
1003002 Biochemistry
0710037 Cancer Biology
0780015 Cell Lines
1002159 Cell Migration
100200A All Biological Sciences
0705025 Digestive System
070500A All Anatomical Systems & Sites
1002017 Embryology
0725005 Environmental Health
072500A All Environment (Health & Safety & Medical)
0765014 Gene Expression
0765015 Gene Regulation
1002019 Genetics
0705040 Immune System
0710070 Immunology
0715129 Mental Disorders
0715135 Metabolic Diseases
0765020 Metabolism
1002059 Morphogenesis
0705050 Musculoskeletal System
0705055 Nervous System
0785100 Neuroanatomy
078500A All Specialties of Medical & Allied Fields
0715138 Neurological Disorders
1002030 Neuroscience
0780025 Organs
0414034 Psychology of Aging
041400A All Psychology
0730070 Public Health
073000A All Health Care
0760075 Receptors
076000A All Molecular & Cellular Entities
0000034 Research
0785000 Specialties of Medical & Allied Fields
0760000 Molecular & Cellular Entities
1002008 Biology, Molecular
1002000 Biological Sciences
1002004 Biology, Cellular
0730000 Health Care
0705000 Anatomical Systems & Sites
1002044 Physiology, Vertebrate
1002053 Human Development
0705015 Cardiovascular System
076500A All Natural Processes (Health & Safety & Medical)
0765000 Natural Processes (Health & Safety & Medical)
1002058 Molecular Genetics
0715165 Pulmonary Diseases
079000A All Structure & Function (Health & Safety & Medical)
0790000 Structure & Function (Health)
0765038 Programmed Cell Death
079500A All Technology Assessment & Transfer & Outreach (Health & Medical)
074500A All Intervention, Types of (Health Safety & Medical)

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