Genomics Resource Lab

Real-Time PCR

Introduction

We offer real-time PCR services to the University of Massachusetts - Amherst, the other institutions of the Five Colleges consortium and the greater Pioneer Valley scientific community.

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Real-time PCR is a technique that allows for the direct monitoring of a PCR reaction as it occurs (hence the term real-time). Real-time PCR instruments have specialized light sources, detectors and optics that measure the emitted fluorescence of labeled PCR product. Because different labeling methods are available, not only can the quantity of the PCR product be measured but other techniques such as melting curve analysis and allelic discrimination are possible.

Real-time PCR is especially accurate for quantifying PCR products since it can detect them in the linear phase of amplification vs the end-point detection typically performed using gels. Moreover, since up to four fluorescent labels can be used at once, more than one product can be detected in the same tube, this is referred to as multiplexing. The former terminology is preferred since it encompasses all aspects of this technology. For additional information on real-time PCR, please see Useful Links.

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